Background: Breast cancer (BC) is the second most prevalent kind of cancer globally and the most common type of cancer among women. Objective: To evaluate the therapeutic efficacy of photo bio-modulation therapy for treatment of neuropathic pain, inflammation and sensation defect in chemotherapy induced peripheral neuropathy in breast cancer patients. Patients and methods: Sixty female patients who had breast cancer and suffered from peripheral neuropathy at least after one cycle of chemotherapy at the National Cancer Institute –Cairo University were included in this study. Both groups of patients were equally divided using a randomization procedure. (thirty patients for each group). Group (A) "Study group": Thirty patients who were given photo bio-modulation therapy (pulsed diode laser) and the routine medical care, Group (B) "control group": Thirty patients who were given the routine medical care only, both before and six weeks following treatment, the participants underwent evaluations using Chemotherapy Induced Peripheral Neuropathy Assessment Tool (CIPNAT) and Visual Analogue Scale (VAS). Results: There was a substantial reduction in CIPNAT as well as VAS of group A contrasted with group B following treatment (p = 0.001) while the percentage of improvement pre and post values of group A in VAS was about 42% and in CIPNAT was about 35% and in Group B was 16% and 19% respectively. Conclusion: There was a detectable improvement in the Chemotherapy-Induced Peripheral Neuropathy Assessment Tool (CIPNAT) of about 22% and VAS about 21% difference in patients treated with photo-biomodulation (PBM) therapy compared to routine medical care. PBM treatment may provide significant symptom benefits in patients with established Chemotherapy induced peripheral neuropathy (CIPN).[