This study aimed to determine the physiological and hematological responses of white shrimp (Litopenaeus vannamei) fed Manna Oligosaccharide (MOS). The experimental design in this study was a completely randomized design (RAL) consisting of four treatments with three replicates, namely treatment A feed without the addition of prebiotics (control), B feed with the addition of 1 % prebiotics, C feed with the addition of 1.5% prebiotics, and D feed with the addition of 2% prebiotics. The test animal used in this study was a white shrimp with a weight of 5-7 grams with a stocking density of 1 head/l. The results of this study showed that feeding treatment with the addition of Mannan oligosaccharide prebiotics (MOS) by 1% could increase the Total Haemocyte Count of white shrimp (76.67 x106 cells/ml), Differential Hemocyte Count (Granular Cells: 39.36%, Hyaline Cells: 38.62%, and Semi granular Cells 22.02% ), and phagocytosis activity (69.95%).