This study evaluates the physicochemical and anti-inflammatory properties of pumpkin peel extract, highlighting its potential applications in pharmaceuticals and herbal formulations. The extract exhibited solid or semi-solid consistency, greenish color, and earthy aroma, with extractive values of approximately 12% w/w for ethanol and 15% w/w for aqueous solutions. Loss on drying was measured at 8.25% w/w, and the extract contained 1.25% w/w of foreign organic matter, indicating its purity and stability. Phytochemical screening revealed a diverse chemical composition. The extract demonstrated significant inhibition of albumin denaturation and heat-induced hemolysis in a concentration-dependent manner, achieving a maximum inhibition rate of 49.25% at 500 µg/ml. In a rat model, both low and high-dose formulations significantly reduced edema, while acute toxicity studies showed no mortality at administered doses of 150 mg/kg and 200 mg/kg. These findings support the extract's therapeutic potential and safety for further preclinical and clinical investigations.