This study evaluated the effects of ethanolic leaf extracts of Melia dubia (EEMD) and Vitex negundo (EEVN) on female reproductive health, particularly in addressing infertility issues. Doses for both extracts were established at 500 mg/kg body weight (bw) per oral administration based on a prior Acute oral toxicity study. In comparison to the ethinyl estradiol (EE) group, the treated groups demonstrated a considerable improvement in estrous cycle regularity over 30 days (P<0.001), approximating the standard treatment group and significantly lowering the metestrus and diestrus phases. There was no harmful effect since hematological indicators such as Hb, RBC, and WBC stayed within normal levels. The extracts were associated with a significant increase in serum estrogen and follicle-stimulating hormone (FSH) levels, suggesting an enhancement in pituitary-ovary axis activity and improved hormonal balance. Body weights remained stable, and histopathological studies confirmed enhanced ovarian and uterine health, with increased ovarian follicles and uterine tissue integrity. Fertility assessments indicated a substantial increase in litter numbers (P<0.001) for the EEMD and EEVN groups, comparable to the standard treatment. Overall, Melia dubia and Vitex negundo extracts show potential in managing female infertility by promoting hormonal balance, estrous cycle regularity, and healthy ovum production.