The objective of this study is to examine the phytochemical properties and biological activities of the aerial parts of the Lagenaria siceraria plant from the Guemmar and Ourmes regions, extracted with methanol. The study demonstrated that the methanolic extracts from the Guemmar and Ourmes regions possess significant phytochemical and antioxidant properties, particularly those from the Guemmar region. It showed a total phenolic compound content of 25.866 ± 0.09 milligrams of gallic acid equivalent per gram of extract, flavonoid content of 7.625 ± 0.120 mg QAE/g Ex, and protein content of 260 mg/g. In comparison, the Ourmes extracts presented a phenolic compound content of 5.43 ± 0.44 mg GAE/g Ex, flavonoid content of 3.418 ± 0.227 mg QAE/g Ex, and protein content of 95 mg/g. Methanolic extract-based creams showed concentration dependent wound healing, with the EG cream inducing nearly complete tissue repair in 20 days. These properties confer a notable therapeutic potential to the plant, particularly in terms of wound healing. Consequently, Lagenaria siceraria could be exploited as a valuable natural resource for nutritional and medicinal applications, allowing us to highlight the wound-healing potential of the plant through creams at different concentrations.