Hyperbilirubinemia is the result of an imbalance between the production and excretion of bilirubin by the liver. Various types of disorders, both acquired and congenital, can cause neonatal hyperbilirubinemia. Bilirubin in high concentration is toxic to the brain because bilirubin can penetrate the blood-brain barrier and might cause irreversible neurological damage. [1] Typically, bilirubin concentration increases within 96-120 hours after birth, peaks on day 5 to 7 and then decreases. The physiological jaundice is caused by increased foetal haemoglobin catabolism that accompanies an immature liver as well as intensified enterohepatic circulation. [2] Monitoring of serum bilirubin concentration is essential during early neonatal life. Approximately 60% of term babies and 85% of preterm babies develop clinically apparent jaundice. [3] Accurate measurement of bilirubin in blood or serum is very important for both diagnostic purposes and therapeutic monitoring of small babies with hyperbilirubinemia. Although bilirubin is one of the most commonly performed laboratory measurements in new-borns, its measurement remains remarkably inaccurate. Common problems faced in neonatal Bilirubin estimation are low sample volume, hemolysis and exposure of sample to light due to delay in transport or analysis.[4,5,6]