Saliva being one of the most commonly found body fluid at the crime scene is an important forensic tool. Saliva, in forensics can be an extremely helpful tool in identifying the age, sex, ABO blood groups, microbial signature.Forensic science continues to flourish, integrating cutting edge technologies to developing proficiencies and aid in criminal investigations. Among these advancements, fluorescent spectroscopy stands out as a powerful benchmarking tool that had gained traction in forensic analysis, particularly in predicting the presence of human saliva, even on inanimate objects and even helps in detection and identification using dried salivary stain too. This article dives into the intricate world of Fluorescence spectroscopy, it’s application in forensic science, and the significance of its role in detecting and analyzing human saliva traces, and, ongoing researches holding promises for further refining this technique, making it an indispensable asset in unravelling mysteries and delivering justice in the realm of forensic science.