Brucellosis, caused by the Brucella bacteria in cattle, has a significant impact on the health and reproduction of livestock, leading to economic and zoonotic consequences in Andean communities. Ecuador is not exempt from this reality. In this context, this study aimed to analyze the prevalence of the disease using the qPCR molecular technique. For this purpose, 50 blood samples were collected from cattle in five communities in the Salinas parish, Bolívar province. Evaluating factors such as age, sex, and gestational status revealed that in the Yacubiana community, 1 out of 14 samples tested positive forBrucella, reflecting a 7.14% prevalence in the analyzed livestock. In Mercedes de Pumín, 1 out of 11 samples tested positive, indicating a 9.09% infection rate in that community. However, Apahua, Verdepamba, and San Vicente de Plancha yielded negative results for the pathogen. In summary, 4% of the specimens analyzed showed positivity for Brucella, emphasizing the urgent need to implement preventive measures and monitoring in these communities to safeguard the health of the bovine population and prevent the spread of the disease in the region.