Climate scientists promote eco-friendly fossil fuel alternatives. Bioethanol from weeds might solve lignocellulosic biomass concerns. This study examined the potential of Chenopodium album for biofuel production. EHG eliminates 18.4-20.2 moles H2 per kg organic carbon. AF and EHG produced 27–33.4 moles (0.5–0.70 dm3/g TOCR). The 48 hour anaerobic fermentation method produced 30.88-45.2 moles of biogas per kilogram of total organic carbon removed (TOCR) by converting acetate and formate (AF → MG). Methane production ranged from 27.34 to 93.02 moles. The three-stage anaerobic fermentation comprising acidogenic fermentation (AF), methanogenesis (MG), and electrohydrogenesis (EHG) produces 81.4% more biogas than the single stage acidogenic fermentation. Highly efficient hydrogen-methane biogas production yielded 35.27 to 45.31 moles per kilogram of TOCR. Thermochemical process converted cellulose into glucose and xylose. Enzymatic biomass breakdown improved cellulose hydrolysis by 95.1%. This method produced 45 g/l glucose. The maximal weed saccharification glucose yield was 93%. Furthermore, biofuel analysis revealed 91% bioethanol and 0.0021 mg/L methanol. The water, Copper and Chlorine content was found to be 0.201%, 0.056 mg/kg and 19.33 mg/l respectively. The gum concentration was 2.1 mg/100 mL. In addition to this, pH of biofuel was found to be 8.8, with 6021 kcal/kg heating value, 0.75 g/cm3 density, 2.11 cSt viscosity, and a flash point of 13. Each reading showed bioethanol levels produced from the Chenopodium album within the permissible limits.