Microvascular anastomosis is a crucial technique in reconstructive surgery. Numerous methods have been developed to enhance vessel patency rates, reduce anastomosis time, and improve hemostasis. Currently, the handheld suture technique is the standard approach for microvascular anastomosis. Traditionally, this involves using simple interrupted sutures after approximating the ends of the vessels. Despite its widespread use, this method can be time-consuming and carries the risk of iatrogenic intimal injury and subsequent vessel thrombosis. Fibrin glue mimics the final stages of the coagulation cascade to form a natural fibrin clot. Several studies have demonstrated the benefits of fibrin glue in microsurgery. Fibrin sealant, also known as fibrin glue, serves as a valuable and versatile tissue adhesive in various surgical contexts. It effectively stops bleeding and ensures air and fluid tightness. Derived from plasma, fibrin glue offers the advantages of biocompatibility and biodegradability without causing inflammation, tissue necrosis, or fibrosis.