Two bacterial strains were isolated from water samples. To check the pathogenicity of isolates, blood agar test was performed. Both isolated bacterial strains showed β hemolysis. Antibiotic susceptibility/resistance test was performed to check the resistance of isolated bacterial strains against different antibiotics. Cefixime, Meronem, Doxycycline, Oxytetracycline, Chloramphenicol, Ciprofloxacin, Amoxicillin and Azomax were used to check antibiotic resistance or susceptibility of isolated bacterial strains. Strain 1 showed resistance against cefixime and strain 2 showed resistance against ceftriaxone. Green synthesized silver nanoparticles and plant extracts of Neem (Azadirachta indica), Calotropis procera, Cassia fistula and Eucalyptus radiata were also used to check the antibacterial activity of isolated bacterial strains. No strain showed resistance against any naonoparticle. Both strains did not show any antibacterial activity against aqueous plant extracts. Molecular characterization or ribotyping results showed that isolated bacterial strains were Escherichia coli and Enterococcus species. The study showed that river Ravi water is suitable for irrigation purpose due to presence of different pathogenic microbes in river water and ultimately cause different infections in humans. Due to overuse of antibiotics bacteria developed resistance against some antibiotics so nanoparticles and plant extracts should be used as antibacterial tools against which bacteria might not developed resistance.