Volume 6 | Issue -15
Volume 6 | Issue -15
Volume 6 | Issue -15
Volume 6 | Issue -15
Volume 6 | Issue -15
Herbalism is one of finest practice used since ancient times to restore, cure and maintain human health. The practice has been commenced with the use of raw unprocessed form of herb and reached its pinnacle in the modern science with the use of pure isolated compound from plant material. The fundamental concern with such plant material and formulations associated with the plants are lack of standardization and safety evaluation. It is sole responsibility of manufacturer to substantiate the efficacy and safety of such product. Development of standardization parameter is tedious task due to presence multitudinous component and their variability in composition. Simultaneous quantification of multiple constituents in single formulation or extract is quite demanding task. The objective of the study was to develop and validate a HPLC method for Simultaneous determination of ten cardinal bioactive compounds i.e. Luteolin, Rutin, Hesperidin, Baicalin, Curcumin, Demethoxycurcumin, Bisdemethoxycurcumin, Piperine, Glycyrrhizin and Quercetin in marketed herbal suspension. The developed method was specific and precise with relative standard deviation < 2.0%. Calibration curves showed excellent linear regressions (R2 > 0.99). The recovery for components in herbal suspension was between 99.2 to 101%. The method is sensitive to quantify all components at minimum concentration viz. Baicalin- 7.3µg/mL,Rutin–15.12µg/mL,Hesperidin–11.38µg/mL, Luteolin-11.86µg/mL, Glycyrrhizin–5.0µg/mL, Quercetin – 29.92µg/mL, Piperine – 4.47µg/mL and Curcuminoids– 8.76 µg/mL. The developed method was accurate, precise, reproducible and appropriate for routine quantitative analysis of stated components.