Tabernaemontana divaricata a common garden plant in tropical countries has been used as a traditional medicine. It has wide-ranging biological activities especially due to the alkaloidal and non-alkaloidal and phytosteroidal constituents. The beneficial properties of Tabernaemontana divaricata are antioxidant, anti-infection, anti-tumour action, analgesia, anti-inflammatory, antifertility and the enhancement of cholinergic activity in both peripheral and central nervous systems. The augmentation of cholinergic function may be of therapeutic benefit for many neurodegenerative diseases, particularly myasthenia gravis and Alzheimer’s disease. During the study leave of the plant are shade dried and converted into coarse powder which further subjected to extraction by soxhlation process using petroleum ether and ethanol. The obtained crude extract further subjected to fractionation and the fractionated extracted was further analysed by different spectroscopy methods.