Plant growth promoting bacteria (PGPR) have been found to be highly beneficial for plants as they help defend against fungal diseases commonly found in soil. They play an important role in plant growth, health and productivity. They increase seedling tolerance to drought, high temperatures, toxic heavy metals, high or low pH and even extreme soil acidity. The present study aim to isolate and characterization of beneficial PGPR for the growth of 17 PGPR isolates, except for isolate 1 and isolate 2 all of them observed a pink color thus indicating binding of iron. Thus, indicating production of Siderophore. All the 17 isolates observed a pink and reddish color thus indicating production of IAA. Most of the isolates showed positive result against ammonia production, phosphate solubilization test and ammonia production. This work highlights the future research works by the use of beneficial strains of PGPR to cultivate rare medicinal plants Mokokchung, Nagaland.