The increased use of antibiotics has led to the development of resistance in many pathogens, representing a major challenge for the treatment of infections. In this study, we explored the bioavailability of selected antibiotics in the presence of nanoscale TiO2 particles prepared from plant extracts. The aim was to determine whether the addition of these nanoparticles could improve the efficacy of existing antibiotics. TiO2 particles are synthesized from plant extracts, making them ecologically sustainable and biocompatible. These nanoparticles were characterized by various techniques,TiO2 nanoparticles was determinedWe got the absorbance at 227 nmwhich is the same as the energy bandgap of commercial TiO2, such as transmission electron microscopy and X-ray diffractionsample showed a dominant peak at 2θ = 26.50˚ and 47˚ which proved the (101) crystallographic plane of anatase and (105) rutile form of TiO2 NPs, to assess their size, shape, and crystal structure Images from Field Emission Electron Microscopy (FE-SEM) verified the nanoparticles’ spherical shape and relatively homogenous size distribution, the EDX spectra clearly demonstrated that the TiO2NPs manufacturing technique is exceedingly safe and pure as no harmful chemicals were utilized. These findings demonstrated that the production of TiO2 nanoparticles was successful, we assessed the bioavailability of some commonly used antibiotics in the presence of these nanoparticles. Bacterial growth inhibition tests were carried out using bacterial strains sensitive to the selected antibiotic. The results showed that antibiotic efficacy was significantly enhanced in the presence of TiO2 nanoparticles dye degradation and bacteriological tests will be performed successively, effectiveness and indicating a promising medicinal performance.