To evaluate in-silico, in-vitro and in-vivo screening of boswelic acid and ferulic acid in Dextran sulfate sodium (DSS) induced ulcerative colitis in Zebra fish model.This Study involves the In silico study by the receptors will be selected for the studies in relation with the Ulcerative Colitis then it is docked by using Autodock 4.2.6 or Autodock Vienna. Then In vitro study is done by using the cell culture where the colorectal derived IEC lines HT-29 will be obtained. Cells were grown with 100 IU/ml penicillin, 100 μg/ml streptomycin, and 10% heat-inactivated FCS in a humidified 5% CO2 atmosphere at 37°C. For signal-transduction experiments Rt-PCR should be performed. In vivo study is performed in the Zebra fish in a separate tank by injecting the Bosewellic acid. The study results suggest that B.serrata and F acid acts by inhibiting lipid peroxidation and its antioxidant effect is significant in the restoration of the enzymes. Thus, the results justify its use for the treatment of gastrointestinal diseases but more detailed studies should be conducted in order to evaluate the protective effect in humans.