This study presents the synthesis and characterization of choline chloride-based choline lauryl sulfate (ChLS) as a novel biocompatible surfactant. The synthesis process involves the reaction between sodium lauryl sulfate and choline chloride in presence of dichloromethane. The resulting ChLS was characterized using various analytical techniques, including Fourier-transform infrared spectroscopy (FTIR), 1H NMR spectroscopy, and surface tension measurements. The FTIR spectrum of ChLS shows significant peaks indicating the presence of cholinium ion and laurylsulfate anion bands. Mass spectrometry confirmed the molecular weight of 265.39 g/mol. Surface tension measurements showed a reduction to 38.7+/- 0.57 mN/m, indicating superior performance compared to sodium dodecyl sulfate (SDS). FTIR, MS and NMR analyses plconfirmed the successful formation of the desired surfactant, while surface tension measurements demonstrated its surfactant properties. The critical aggregation concentration (CAC) was determined to be 0.8 mM, demonstrating efficient micelle formation. Thermal analysis revealed stability up to 256.3°C (Tdecomposition), with decomposition starting at 256.3°C. These findings suggest ChLS as an effective, thermally stable surfactant suitable for various industrial applications. The synthesized ChLS exhibited promising surface-active properties and enhanced biodegradability, making it a potential candidate for various industrial and cosmetic applications. This study contributes to the development of sustainable surfactants with reduced environmental impact.