Six novel pyrimidinone derivatives (IIIa - IIIf) were synthesized by conventional method. The newly synthesized derivatives were characterized by spectroscopical methods using IR, 13C-NMR spectroscopy and Mass spectrometry. All the synthesized compounds were screened for their antibacterial and antifungal activity bycup-plate-agar-diffusion method. The antibacterial activity screening revels hat the compound IIIb has comparable activity and compound IIIc shows moderate activity as that of standard ampicillin against gram positive and gram-negative bacteria. All synthesized compounds IIIc were found to be active as antifungal against Candida albicans