Treatment of 1-acetyl-4-(4-hydroxy phenyl) piperazine with aromatic or substituted aromatic aldehydes in presence of methanol and potassium hydroxide, formed 3-substituted phenyl-1--(4-(4- hydroxyl phenyl)- piperzin-1-yl)-Prop-2-en-1-one derivatives (RC-1 to RC-10). These are assayed for their antibacterial activity against Bacillus pumilus, Bacillus subtilis, Escherichia coli, and Proteus vulgaris; for antifungal activity against Aspergillus niger, Candida albicans strains. Antibacterial assay revealed that Bacillus subtilis and Proteus vulgaris were the most sensitive bacterial strains to compounds RC-2, 3, 4, 7, 10 and in the antifungal assay, compounds RC-1, 3, and 10 were highly effective against Aspergillus niger when compared to other investigated strains.