Background:Anxiety generally impacts all the body's systems, including the cardiovascular, pulmonary, nervous, muscular, and reproductive systems. Aim: To address how anxiety levels among physical therapy students of various study years relate to changes in heart rate and blood pressure throughout various exams, depending on the difficulty of the subject and whether this influences their level of worry. Subjects and methods: This was a longitudinal study looking at how PT students' blood pressure and heart rates are affected by their anxiety before and after exams in varied materials based on the difficulty level.The subjects were healthy physical therapy students attending Kuwait University, and their age range was between 18 and 24 years. The sample included 60 students. 20 students were selected from each academic year of study. Results: There were significant differences in systolic blood pressure before and after difficult and easy exams p<0.05, with the third-year group having the highest differences. However, there were no significant differences in heart rate changes before or after exams p>0.05. There were no significant differences in heart rate changes between the three student groups, regardless of exam difficulty level or year of study p>0.05. Conclusion: Readjusting the curriculum to minimize the overload on the students, create a relaxed examination environment, and teach the students better ways to cope with stress prior to each exam. Mentor the students' psychological and mental health by having an adviser office that they can reach out for.