In conventional medicine, the plant Pulicaria crispa is used for colds, cough, colic, excessive sweating, and carminative disorders. Our study aimed to evaluate the biological properties of extracts with different polarities, specifically chloroform (CHCl₃), ethyl acetate (EtOAc), and n-butanol (n-BuOH) extracts derived from Pulicaria crispa (Forssk.) Oliv (P. crispa). The chemical profile of the CHCl3 was determined by Gas chromatography coupled to mass spectrometry (GC-MS), and the biological activities of P. crispa extracts were evaluated regarding antioxidant, anti glycation and anti acetylcholinesterase effects. Our findings represent the first record about the CHCl3 extract that showed the presence of 35 different compounds with three major ones, we name: 2,5 Dihydroxynaphthalene-1,4-dione, 14-methyl Pentadecanoic acid methyl ester and Hexadecane with percentage area of 16.19%, 12.37% and 8.99%, respectively. Furthermore, the biological investigation demonstrated that P. crispa extracts possess significant antioxidant properties and, for the first time, revealed their anti acetylcholinesterase and anti-glycation activities. Notably, the EtOAc extract exhibited the highest antiglycation potency, surpassing even the standard reference compound. Authors claimed that further surveys are required to isolate major components and conduct in vivo and clinical trials to validate their potential as therapeutic agents.