Objective: To evaluate the impact of Transurethral Resection of Prostate (TURP) on Sexual Function. Design: A quasi-experimental study. Place and duration of study: conducted from 1/9/2021 to 31/12/2023 at The Kidney Centre, Karachi, Pakistan. Methods: Total of 37 patients aged between 50–80 years were evaluated for the effects of TURP on sexual function. Pre- and post-operative sexual function was assessed using the International Index of Erectile Function (IIEF-15). Statistical analyses included paired t-tests, Wilcoxon sign-rank tests, and regression analysis to explore factors influencing changes in sexual function. Results: Post-TURP, IIEF-15 scores showed a significant decline (54.8 ± 15.8 pre-op to 40.9 ± 18.4 post-op, p < 0.001). Diabetic patients experienced a more pronounced reduction in scores compared to non-diabetics (23.3 ± 20.1 vs. 10.4 ± 15, p = 0.028). Age negatively correlated with changes in orgasmic function and overall sexual performance, though not significantly. Declines were observed across all IIEF-15 components, including erectile function, orgasmic function, sexual desire, and intercourse satisfaction. Conclusions: TURP significantly impacts sexual function, with diabetics and older patients showing greater vulnerability. Preoperative counseling about potential sexual outcomes is essential. Further research with larger cohorts is recommended to better understand these effects and optimize treatment strategies