Women between the ages of 45 and 55 go through a phase known as menopause. Menstruation ceases for at least 12 months in those who experience menopause because of a decrease in ovarian function that lowers estrogen levels. Reduced estrogen levels can cause poor fat metabolism, which can lead to osteoporosis and atherosclerosis. The purpose of this study was to demonstrate the effect of a flavonoid extract obtained from P. macrocarpa on the diameter expansion of the aorta and the rise in the number of osteocytes in the femoral bone of menopausal mice. This study utilized a genuine experimental laboratory setting with a research design of Randomized Post Test Only Control Group setting. Using 32 female mice divided into 6 groups: K- (without ovariectomy and flavonoid extract P.macrocarpa), K + (ovariectomy without treatment), P1 (ovariectomy + dose 3.75 mg/mice/day), P2 (ovariectomy + dose 7.5 mg /mice/day), P3 (ovariectomy + dose 11.25 mg/mice/day), and P4 (ovariectomy + dose 15 mg/mice/day). Administration of flavonoid extract P.macrocarpa was carried out for 14 days. Examination of Caspase 3 expression using the Immunofluorescence Data analysis method using SPSS 27.0. The results showed that in a post-hoc test, namely the administration of P.macrocarpa flavonoid extract at a dose of 11.25 mg/mice/day and 15 mg/mice/day showed that it could increase the number of osteocytes of the femoral bone of mice model of menopause. Based on the findings, it can be inferred that administering P.macrocarpa extract has a notable effect. Giving flavonoids from Phaleria macrocarpa fruit extract can reduce the expression of Caspase 3 and increase the number of osteocytes in the femoral bone of menopausal mice