Background: Adolescents with visual impairment face challenges in obtaining reproductive health information. The use of mobile applications is one alternative that can be used to improve information accessibility by providing interactive and disability friendly educational materials. This study aims to analyze the effect of mobile applications on reproductive health literacy of adolescent girls with visual impairment and compare the results in the control and intervention groups. Methods: This type of research used a quasi experiment dengan desain pre and posttest control group design. The sample was 230 people divided into control and intervention groups of 115 people each using total sampling. Data collection methods using standardized instruments, namely Sexual Reproductive Health Literacy (SRHL) for sexual reproductive health literacy variables. Statistical analysis used paired sample t-test and independent sample t-test. Results: The results showed that in the intervention group results of reproductive health literacy pre-test is 43.20 and post-test is 73.61. Meanwhile in the control group results of reproductive health literacy pre-test is 43.14 and post-test is 48.19. There was a significant difference between the control and intervention groups on the average reproductive health literacy (p=0.000) where the increase in scores in the intervention group was greater than the control group. Conclusion: The results also show that there is an effect of mobile application on reproductive health literacy among adolescent girls with visual impairment in the intervention group, in addition results also showed that there was a difference in reproductive health literacy among adolescent girls with visual impairment in the control and intervention groups.