Aim: The purpose of periodontal therapy is to halt the increasing deterioration by managing infection, hence preventing tooth loss. This study was to determine if a combination of strontium chloride membranes, hyaluronan, and chitosan would be beneficial in treating periodontal intrabony defect. Methods: This randomized controlled trial included thirty six patients, ranging in age from twenty-five to fifty-five, participated in this study, they were split into two equal groups: Group B (Test group): patients received surgical treatment using hyaluronan, chitosan, and strontium chloride membrane; Group A (Control group) patients had open flap debridement. Results: Group B experienced significantly less pain than group A at D1 and D2 (P<0.001), but the remaining durations were similar between the two groups. Groups A and B showed significant decreases in gingival index and probing depth at 3 and 6 months compared to baseline (P<0.001). Conclusions: When addressing intrabony defects, the triphasic Hyaluronan-Chitosan-Strontium Chloride membrane was used in combination with periodontal flap surgery; nevertheless, there was no statistically significant change observed when compared to OFD alone. Both groups showed improvement in terms of radiographic bone fill, gingival index, clinical attachment loss, and periodontal probing depth. Furthermore, as evidenced by decreased discomfort and swelling, the tissue's reaction to the triphasic Hyaluronan- Chitosan-Strontium Chloride membrane was positive