The objective of this study was to assess the immunomodulatory effects of Eleusine indica in Wistar albino rats with cyclophosphamide-induced immunosuppression. Wistar albino rats were divided into five groups, with each group comprising six rats. Group I served as the normal control, Group II received cyclophosphamide (20 mg/kg, i.p.) for 10 consecutive days, Group III received cyclophosphamide along with the standard drug levamisole (50 mg/kg, p.o.) for 10 consecutive days, Group IV received cyclophosphamide along with Eleusine indica extract (200 mg/kg, p.o.) for 10 consecutive days, and Group V received only Eleusine indica extract (200 mg/kg, p.o.) for 10 consecutive days. Following the treatment period, various immunological parameters such as hematological parameters, humoral immune response, and cell-mediated immune response were assessed. Cyclophosphamide administration led to a significant reduction in hematological parameters, humoral immune response, and cell-mediated immune response. However, treatment with Eleusine indica extract significantly mitigated these cyclophosphamide-induced alterations. Additionally, Eleusine indica extract exhibited immunostimulatory effects in normal rats. The findings of this study suggest that Eleusine indica possesses notable immunomodulatory properties and could serve as an adjunctive therapy in immunocompromised conditions.