This study investigates the barriers and motivators involved in blood donation among medical and non-medical students in private institutions. Analysis of data from 675 nonmedical students and 894 medical students reveals that 31.8% of medical students have donated blood, while 27.11% of non-medical students have done so. Primary motivators for non-medical students include the opportunity to save lives (17.48%) and contribute to society (6.22%), whereas medical students are primarily driven by saving lives (21.47%) and societal contribution (5.92%). Barriers such as inconvenient donation center locations (12.34% for non-medical, 10.67% for medical), eligibility uncertainties (8.91% for non-medical, 9.24% for medical), academic commitments (5.67% for nonmedical, 6.81% for medical), and psychological concerns (3.45% for non-medical, 4.12% for medical) hinder participation. The findings underscore the necessity for targeted awareness and educational programs to boost blood donation rates among both student groups. Understanding these factors is essential for fostering a culture of regular blood donation among students in educational environments.