This study aims to investigate the impact of sleep deprivation on stress coping in both medical and non-medical students. Getting enough sleep is crucial for overall health. In academic contexts, sleep deprivation is a prevalent issue, particularly among medical students who have been trained more extensively than their counterparts. There is rising worry regarding its effect on stress-coping strategies. Materials and Methods: 120 undergraduate students are alienated from medical and non-medical disciplines. The Pittsburgh Sleep Quality Index (PSQI), Perceived Stress Scale (PSS) and Brief COPE questionnaires were used to evaluate sleep quality, level of stress and stress coping strategies, respectively. Result: Current study findings suggest poor quality of sleep in medical students and slightly higher levels of stress in non-medical students compared to medical students. However, coping strategies remain similar across both groups. Gender differences indicate that significant gender disparities in quality of sleep, stress levels and stress coping strategies in both medical and non-medical groups, although statistical significance is lacking. Females reported higher stress levels, especially in the non-medical group and more frequent use of Emotion-Focused Coping compared to males in both medical and non-medical groups, whereas males reported slightly better quality of sleep in both medical and non-medical counterparts. Conclusion: The study emphasizes the need for specific interventions to reduce stress and improve sleep quality, promoting healthy coping strategies and sleep hygiene in both medical and non-medical groups. More studies to understand the significance of sleep quality are desired, particularly in students’ lives.