This study was conducted in Algeria (case of Ghardaia). Its primary objective is to investigate the biodiversity of aphid parasitoids on both cultivated and non-cultivated plants in the Ghardaia region and to identify the various tritrophic relationships. This study allowed us to identify eight species of primary parasitoids: Lysiphlebus fabarum, Aphidius matricariae, Aphidius ervi, Aphidius colemani, Aphidius funebris, Trioxys angelicae, and Praon volucre. The last two species are reported for the first time. One unidentified hyperparasitoid species was found to be active on the larvae of primary parasitoids. In terms of associations (plant- aphid- primary parasitoid), we identified 23 associations.