Background: Chemo-mechanical preparation is a crucial component of an effective endodontic treatment plan. It is accomplished by the tools and efficient irrigation products. Irrigating solutions are used to dissolve organic and necrotic tissue, avoid dentin chip packing in the apical region, and eliminate smear layers and debris. Healing potential of plants is an age old idea that has recently attained renewed interest. Considering the ineffectiveness, potentially harmful effects, and safety concerns of commonly used synthetic irrigants, the herbal alternatives for endodontic usage might prove to be advantageous. The aim of this study was to compare of smear layer removal efficacy of Neem, Triphala and 17 % EDTA as root canal irrigants. Materials and Methodology: thirty human mandibular first premolars were decoronated to a root length of 17mm. They were cleaned and shaped upto 40k size file using saline as an irrigant throughout the instrumentation. The specimens were equally divided into 3 groups according to final irrigant used for smear layer evaluation; group 1- Neem solution, group 2 -Triphala solution, group3-17% EDTA. Samples were split longitudinally and examined under microscope for smear layer evaluation at coronal, middle and apical levels. Results: Triphala demonstrated the maximum efficiency in eliminating the smear layer than EDTA and Neem solution. Conclusion: Neem and Triphala both showed the potential to eliminate the smear layer. Triphala demonstrated the maximum efficiency in eliminating the smear layer.