ISSN : 2663-2187

EXPLORATION OF INDIGOFERA PROSTRATE AGAINST OXIDATIVE STRESS AND EVALUATION FOR NEUROPROTECTION IN CHEMICALLY INDUCED NEUROTOXIC RATS

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Neelam Injeti, Kumar Shiva Gubbiyappa
» doi: 10.33472/AFJBS.7.1.2025.17-36

Abstract

The goal of the current study was to assess the effect of Indigofera prostrate on memory and spatial learning induced by a Bisphenol A (BPA) in experimental rats and how I. prostrate helps to mitigate these effects. Methods: The methanolic seed extract of I. prostrate was prepared, and subjected to phytochemical constituents’ analysis. Wistar Albino rats weighing 150 + grams were divided into 5 groups –Control, positive control, standard drug and test drug treated groups (200 & 400 mg/kg). In vitro and in vivo anti-oxidant methods were employed to evaluate the anti-oxidant property of I. prostrate. The behavioural paradigm of animals was analysed by using models like elevated plus maze, Y maze and actophotometer. At the end of the experiment, the brain tissue was isolated and evaluated for anti-oxidant enzymes. Results: The in vitro anti-oxidant enzyme activity was evaluated by DPPH, ABTS and metal chelating assay and the free radical scavenging property of the test extract was proven with the IC50 values and the % inhibition. There was an improved behavioural pattern with the treatment of the test drug in the above-mentioned models, all the values were found to be significant (**p<0.001). Furthermore, when the oxidative stress and anti-oxidant level was assessed by biochemical tests like MDA (Malondialdehyde), SOD (Superoxide dismutase) and catalase, there was an increased levels of anti-oxidant enzymes in a significant manner (**p<0.001). Conclusions: It was concluded that I. prostrate showed a remarkable effect on behavioural pattern, exhibited anti-oxidant effect on oxidative stress induced by BPA.

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