Children's visual development is influenced by a multitude of environmental and lifestyle factors, underscoring the importance of understanding disparities in visual characteristics between urban and rural settings. This study sought to compare stereo acuity, amplitude of accommodation, and refractive status in school-aged children (6-12 years) from urban and rural areas in Pune district. A total of 900 school children from both urban and rural schools were systematically selected for evaluation. Measurements included stereo acuity using the Titmus stereo test, amplitude of accommodation using the RAF rule, and refractive status with full spectacle correction. Statistical analysis, including descriptive statistics and chi-square tests, was employed. The demographic study encompassed participants from 7 Taluka schools, with the highest number hailing from Maval Taluka. Significant differences in stereo acuity and amplitude of accommodation were noted between urban and rural children. Interestingly, stereoacuity was notably lower compared to refractive errors and amplitude of accommodation. This discrepancy in stereo acuity highlights potential environmental influences on visual development, suggesting the need for early detection and management of visual issues. Recognizing these variations can inform the implementation of targeted public health initiatives and vision care strategies tailored to the specific needs of urban and rural populations, ultimately enhancing academic performance and overall well-being among children