Introduction: Organ donation is a vital process that saves many lives each year by providing organs and tissues to those in need of transplants.This process typically requires consent from the donor while they are alive or from their next of kin after death. Aim: To determine knowledge and attitude regarding organ donation among nursing students at selected nursing colleges of kheda-anand district. Methodology:The non-experimental descriptive research design used for this study. The study was conducted on 250 nursing students from selected nursing colleges of kheda-anand district by systematic random sampling technique. A knowledge questionnaire tool and likert attitude scale was used for data collection. Results:1.6% students having good knowledge, 30.4% having average knowledge and 68%having poor knowledge regarding organ donation. The mean score of knowledge is 12.132±4.363. when 70% students having positive attitude, 30% having negative attitude regarding organ donation. The mean score of attitude is 65.67±9.168. Conclusion:The study highlights the disparity between knowledge and attitude regarding organ donation among students. Although the majority lack adequate knowledge, a considerable number still maintain a positive attitude. This emphasizes the need for targeted educational interventions to improve understanding and promote positive attitudes toward organ donation.