The present study demonstrates that amino acids and isocyanate form a cross-linked polymer network at room temperature. The amine group in the amino acid acts as a hardener during curing. These results offer several possibilities for applications in important fields that require thermoset materials with toughness, stiffness, and other coating properties. The composites exhibit effective antifungal and positive antibacterial activity against certain strains. TY-TDI and TH-IPDA composites show a significant zone of inhibition compared to standard Amoxicillin. The scanning electron microscopy analysis confirmed the successful formation of the nanocomposites. The synthesized nanocomposites show improvements in characteristics like pencil hardness, scratch resistance, gloss, cross-cut adhesion, and flexibility. Finally, the execution of a chemical reaction between two phase materials was utilized as a modified coating. This chemically activated nanocomposite was integrated into the core structure of the polymer matrix to deliver better surface and mechanical properties.