It is essential to increase the stability of the enzyme.The activity of the enzyme is also required to be maintained. In this paper, these were achievedthrough immobilization techniques. Adsorption on nanoparticleswas used for this purpose. Glucose oxidase (GOx) and horseradish peroxidase (HRP) were studied for this purpose. ZnO nanoparticles were synthesized via a green method for the immobilization process. The research focuses on standardization techniques to optimize various parameters, including temperature, pH, concentration, and incubation time, in enzyme immobilization. The study concludes with a comparative analysis between green synthesized ZnO nanoparticles and commercially available native ZnOnanoparticles.