The proteins are essential for normal physiological functioning and maintaining the homeostasis. To know about general health the serum albumin, serum globulin and albumin-to-globulin ratio (AGR) are considered as the good parameters. These markers also help to assess the immune functioning and (AGR) is a prognostic factor for many medical conditions. To understand the effects of a novel multiherbal formulation (AKSS16-LIV01) prepared on this aspect of the health experiments are done with CCl4 treatment and administration of the formulation. This CCl4 is also considered as an environmental toxicant which also broadens the implications of this study. From results of the experiments, it has been seen that administration of the formulation significantly lowered elevated albumin and globulin levels and normalised the albumin-to-globulin ratio. It can be inferred from the study that the formulation has potent effects on maintaining the levels of the serum marker proteins, which implicates the healing effects of the formulation. It can be due to synergistic effects of the molecules present in the constituents of the formulation.Multiherbal formulation, albumin, globulin, albumin-to-globulin ratio (AGR), CCl4