The perennial herb Stevia rebaudiana, which belongs to the Asteraceae family, is a well-liked natural sweetener that performs better than traditional high-potency sweeteners in terms of both sensory and functional aspects. Sweet glycosides found in stevia rebaudiana leaves have physiologic effects. Measurements of the other glycosides present in extracts are necessary since, besides the main glycosides, they could potentially be involved in activity. The identity of dulcoside A, steviolbioside, rebaudioside C, and rebaudioside B was confirmed using the isocratic HPLC technique. The International Conferences on Harmonisation (ICH) was utilised inthe creation and approval of the HPLC method for the concurrent evaluation of steviolbioside, stevioside, and rebaudioside-A (harmonisation) standards in Stevia rebaudiana leaves. Using liquid chromatography with high (High-performance liquid ) as the reference method, Utilising near-infrared reflectance spectroscopy (NIRS), the amount of glycosides of (SGs that are) percentage (weighted average drying basis in Stevia leaves was measured. Steviol-glycosides are calorie-dense, noncariogenic sweeten found in stevia rebaudiana stems, which may be beneficial to human health. Animal studies have revealed that the aglycone steviol can have unfavourable effects, even though steviol-glycosides are typically thought to be harmless. Stevioside were identified by using ultra-high performance liquid chromatography coupled with electrospray the ionisation mass chromatography (UHPLC-MS) and 2D NMR methods. Next, an internal standard was used to measure the steviol glycosides using NMR of anthracene.