Cellulose is a key structural component in the cell wall of plants and a complex polysaccharide. This review delves into the diverse array of microbial Marvels that possess the enzymatic system to digest cellulose. Through isolation and characterization, likely isolates were identified for their enzymatic activities crucial in decomposition of cellulose. Partial purification of enzymes was achieved through Ammonium sulphate precipitation. Enzyme activity was assessed using the Dinitro salicylic acid (DNS) method for the presence of enzymes, which is commonly utilized for estimating reducing sugars. The fermentation process employed yeast, specifically Saccharomyces cerevisiae culture, resulting in the production of bioethanol. Through intricate metabolic pathways and synergistic interactions, these microorganisms not only facilitate the production of biofuels like ethanol but also offer a sustainable approach for waste degradation such as paper and textile waste. By integrating microbial ecology with bioprocess engineering, the comprehensive analysis underscores the transformative potential, emphasizing the imperative role of Cellulolytic Crusaders in shaping a greener future. In conclusion bio prospecting from widely existing organic material opens doors to innovative approaches in both waste management and renewable energy production.