Background: Umbilical cord blood is considered a valuable resource for potential medical treatments, as it contains a rich supply of stem cells. These stem cells can be sourced from both the cord blood and the cord tissue. This biological material offers a form of insurance against various diseases, providing a promising avenue for future medical interventions.Methodology: A descriptive study was conducted to evaluate the knowledge and attitudes towards umbilical cord blood banking among antenatal mothers in primary health centre , Kelambakkam. This study used purposive sampling technique . Data were gathered using a self-administered, pre- designed questionnaire. Total sample size was 100 were selected in this study . The collected data were then analyzed using the SPSS software program version 27.0 .Results: The study results revealed that the maximum score for knowledge was obtained by those with moderate knowledge (48%), while the minimum score was obtained by those with adequate knowledge (11%). Regarding attitude, the maximum score was achieved by those with adequate attitudes (54%), and the minimum score was obtained by those with severe attitudes (11%). The assessment of knowledge and attitude concerning umbilical cord blood banking among antenatal mothers showed a statistically positive correlation (0.103). The results indicate a significant association between knowledge and various demographic variables such as age, religion, education, language, occupation, type of residence, access to health services, trimester, and source of information. However, there was no significant association between knowledge and monthly income. Similarly, there was a significant association between attitude and demographic variables including age, religion, education, language, occupation, type of residence, access to health services, trimester, and source of information. No significant association was found between attitude and monthly income. Therefore, the hypothesis H1 is statistically accepted.