This paper aims to review the advances made in the field of Biotechnology that have enhanced the nutrient status of the soil especially nitrogen and phosphorus nutrients concerning biofertilizers, GMOs, and microbial inoculants. This study seeks to establish how the following biotechnological applications can be used to improve the quality of the soil and production of crops in agriculture without adversely affecting the sustainability of the approaches used. In conducting the study, the research approach included the analysis of the literature and the assessments of the recent studies that probed into the relationship between biofertilizers, GMOs, and microbial inoculants on the nutrient status and fertility of the soil. Further, the study established how these biotechnological solutions could be incorporated into conventional farming practices regarding aspects such as soil biomes and ecological stability. The studies reveal that the use of biofertilizers like nitrogen-fixing bacteria and phosphorus-solubilizing fungi helps in decreasing the use of chemical fertilizers because they increase nutrient use efficiency. Some of the information gathered about GMOs is that they enhance the efficiency of nutrient utilization and prevent the wastage of nutrients. Also, plant growth-promoting rhizobacteria and mycorrhizal fungi facilitate the decomposition of organic matter and the availability of nutrients to the plant. The biotechnological activities improve the quality and efficiency of soil structure in supporting sustainable agriculture production. Thus, it is possible to conclude that the interaction between the methods of molecular biology and the traditional approaches to agriculture, the definition of the crop-soil relationships, and the impact of the mentioned practices on the soil will define further perspectives of sustainable soil and food production. However, there are some areas to focus on to increase the efficiency of biotechnology in agriculture