Background: The dyslipidemia and hyperhomocysteinemia are the major risk factors for cardiovascular disease (CVD) and stroke patients. Hyperhomocysteinemia induces thrombogenicity and promotes the proliferation of smooth muscle cells, which could lead to vascular constriction and stiffness are well documented in literature. Aim and Objectives: The main aim of our study to see the serum homocysteine and lipid profile levels in stroke patients of our hospital. Material and Methods: Thirty stroke patients admitted to medicine ward of Krishna hospital, Karad (Maharashtra), were included and compared with same age and sex matched healthy controls. Results: Serum homocysteine level (191.8%), ratios of total cholesterol/High density lipoprotein (TC/HDL) (27.08%), and low density lipoprotein /High density lipoprotein (LDL/HDL) (32.66%) were significantly increased in stroke patients as compared to controls. HDL (-17.1%) was significantly decreased in stroke patients as compared to controls. Serum Triglycerides (TG) level (7.18%), Very low density lipoprotein VLDL (8.46%), TC (2.02%) and LDL (10.28%), were not significantly increased in stroke patients as compared to controls. Conclusions: It concludes that the increased serum homocysteine level in this study might be possible cause for the stroke. Along with lipid profile parameters the estimation of homocysteine is also now days beneficial to find out the risk of stroke.