The effects of ALK-F AV (Alkaloid fraction of Adathodavasica) on lipopolysaccharide (LPS) stimulated oxidative damage in cultured human umbilical vein endothelial cells (HUVECs) were studied. The protective effects were assessed using MTT and measuring the generation of reactive oxygen species (ROS). The pro-angiogenic activity of ALK-F AV was tested using in vitro Matrigel tube forming assay and Western Blot. Transwell assay and scratch assay observed the effects of ALK-F AV on the migration of human umbilical HUVECs. Our results proved that ALK-F AV facilitated the proliferation of HUVEC, which was otherwise suppressed by exposure to LPS. ALK-F AV reduced LPS-induced inflammatory mediators and facilitated the establishment of a capillary network. Taken together, the present data indicate that ALK-F AV has attenuated the vascular injury induced by LPS. Thus, promoting angiogenesis through a process that extends beyond the direct delivery of angiogenic factors by up-regulating MMPs expression in endothelial cells