Blumea mollis belonging to the family Asteraceae and the plant has been widely used in traditional medicinal system for the cure of various diseases. It is used by people for the treatment of fever, inflammation and infections. This plant has antioxidant, analgesic cytotoxic and antibacterial property. Materials and Methods: Plant powder extracted with different solvent i.e. Pet. Ether, Ethyl acetate, chloroform, ethanolic, and aqueous as per increasing polarity and extracts of the plant were prepared and analysed. The fluorescence analysis of powdered material was subjected to analysis under long ultra violet light after treatment with various chemical and organic reagents. Analgesic activity will be performed according to the Hot plate model and Tail flick model. Results: The plant powder showed different fluorescence effects in short and long UV light and noted. The present study aimed to explore the analgesic activity of dried ethanolic extract of Blumea mollis (BM) using tail flick, hot plate method. Results demonstrated that BMEE exhibited a potent dose dependent analgesic activity in all tested models for analgesia Conclusion: The results of the present study revealed that the fluorescence analysis of Blumea mollis can be helpful for the standardization and quality control of indigenous drug and plant good source of secondary metabolite. The BMEE extract was found to reduce pain and BMEE extract of Blumea mollis was found to have good analgesic activity.