The Polyscias genus belongs to the Araliaceae family and comprises over 116 species. This genus is grown in the Pacific and Southeastern Asian countries, and many of them possess great medicinal value. However, some Polyscias species in Vietnam are now endangered or threatened with extinction due to the high demand for medicinal use. The most popular Polyscias species, named "Dinh lang"(Ming aralia), have been largely cultivated for medicinal and ornamental purposes. Therefore, there is a need to develop both morphological and molecular markers to accurately classification. In this study, we collected 23 samples of the Polyscias J.R. Forst genus. & G. Forst. (Araliaceae) from 8 different regions (northern, central highland, and south-central coasts) in this country. All samples were characterized using morphological characteristics and ITS markers. As a result, 21 different phenotypes, 3 subspecies and 4 species were identified. Cluster analyses using similarity coefficients ranging from 98.38% to 100% showed that the 23 samples of Polyscias formed 4 major clusters with 11 genotypes. In general, morphological differences (mainly on leaves) and genetic characteristics using ITS markers were consistent with each other, and every genotype included one or more phenotypes. The study also identified the short keys to species and varieties for some Polyscias species growing in this country.