Due to the lack of studies in the field of biodiversity in Libya, especially in ‎animal ecology, this paper aims to assess the diversity and distribution of wildlife animals in three areas of the EL-Jabal EL-Akhdar region of northern-eastern Libya (Jeera, Taknes, Wadi Zaza) and the anthropogenic pressure in these areas, the areas were divided into three squares of 6 km by 12 km covering 72 km2 each, waste remnants, animal remains including feathers and hair, and track traces were used to identify the wild animals, also questionnaire was used to assess the anthropogenic stress in the area, ecological indexes such ‎as Shannon, Simpson, Richness, and Evenness.., among these species that suffer from high risk, Testudo qraeca due to mass exportation ‎and Hayena hyaena L, canis anthus, Vulpes vulpes, subjected to poaching for the trading of their skin.‎These species are associated with the vegetation in the zone of study, especially ‎Juniperus phoenicia, which is considered to be a dominant species in the study area which ‎, in turn, suffers from environmental imbalance.‎ The dense vegetation in the Wadi Zaza area has an impact on the fauna in the form ‎of high rates of animal diversity, especially birds. The study concluded that several animal species are on the brink of ‎extinction due to the intensity of anthropogenic in the different study zones, which ‎ranged from extreme trade and mass exportation to hunting, urban and agricultural ‎expansion, and deforestation Urgent action is needed to stop ‎environmental deterioration, especially land use, and to establish protected areas.‎