The Present study focuses on the efficacy of silver nanoparticles biosynthesized using the aqueous leaf extract of Sphaeranthus amaranthoides on Candida albicans ATCC 10231 and its biofilms. Methods: Silver nanoparticles of Sphaeranthus amaranthoides were synthesized and characterized by UV, FT-IR, Zeta Potential, and SEM. Anti-Candida efficacy of synthesized silver nanoparticles was assessed on Candida albicans by disc diffusion and Minimum inhibitory concentration method. The biofilm formation inhibition effect of Sphaeranthus amaranthoides nanoparticles was assessed. Further mechanistic studies were also studied by evaluating the cell wall components of Candida albicans and its virulence factor like secreted aspartyl proteinase assay. Results: Sphaeranthus amaranthoides silver nanoparticles formation was characterized. The in-vitro disc diffusion method showed a zone of inhibition of 12±0.15 mm at 100 µg/mL concentration. Further mechanistic studies revealed that the biosynthesis of silver nanoparticles of Sphaeranthus amaranthoides acted on the cell wall of Candida thereby breaking the integrity of glucan moiety and proteins present in the cell wall. It also can inhibit the virulence factor such as Secreted Aspartyl Proteinase Conclusion: It is evident that silver nanoparticles of Sphaeranthus amaranthoides have anti-candida potency against Candida albicans and can be used as a therapeutic to treat candidiasis