Biofilm formation and bacterial growth are crucial for the retention of oral health. Several plants like Salvadora persica also known as miswak and Andrographis paniculatahave been used for oral hygiene since ancient times. Newer research suggests better for their extracts in which both types manage to present high bactericidal and anti-biofilm efficiency. The objective of this study was to prepare and characterize a new oral-herbal mouthwash formula that can be beneficial on bacteriostatic, biofilm breakdown & inflammation in the context of avoiding adverse effects. The mouthwash formulation containing the plant extracts were evaluated for their efficacy through different assays. This entailed the examination of their antibacterial potential towards typical oral pathogens and biofilm inhibitory properties. Further, the denaturation activity was studied using Bovine Serum Albumin (BSA) and Egg Albumin (EA) protein by employing Protein Denaturation constants with little unintended side effects of dual cavity-binding.