New drugs for anti-inflammatory, anticancer research is emerging area to work using the medicinal plants and their active phytochemicals, among these already reported for potential property against various cancers and other disorders as a novel natural agent. The current investigation aimed to assess the phytochemicals, anti-inflammatory, anticancer properties using cell lines of various extracts of the Indian traditional medicinal plant Indigofera cardifolia aerial parts. To determine the anti-inflammatory activity, protein denaturation assay carried out using Aspirin standard drug and three extract of aerial parts of the I. cardifolia. In anticancer property using MTT (3-(4, 5-dimethythiazolyl 21-2 5-dphenyttetrazolum bromide) in vitro cell proliferation assay for all the three extracts and screened qualitatively phytochemicals as per the Harnborne method to know the potential secondary metabolites. The anti-inflammatory property found potent in water extract by percent inhibition of protein denaturation showing IC50 value 92.97µg/ml compared to control Aspirin value 50.34 µg/ml. In anticancer studies also found potential water extract in in vitro proliferation MTT assay using MCF-7 cell lines by showing IC50 value 98.56µg/ml when compared to that of Cisplatin standard. The active key agents in the all the extract found positive result for phenols, glycosides, saponins, steroids, terpenoids and flavonoids in water extract only and other two extract shown positive for phenols and alkaloids. The results of this study conclude that I. cardifolia aerial parts possess the strong anti-inflammatory and anticancer potential which are emphasized by the presence active phytochemicals to be considered as active therapeutic agents.