In reaction to the macro and microenvironment, the fish skin mucus has a unique immune role. The goal of the current study was to characterise the biochemical properties of the epidermal mucus of the freshwater striped snakehead Channa striata L. as well as its antibacterial capacity. In order to do this, epidermal mucus samples were collected and tested for their ability to inhibit the growth of four pathogenic bacterial species, including Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli. According to the findings, C. striata mucus had a stronger antibacterial impact against Pseudomonas aeruginosa and Escherichia coli. Results of GC-MS analysis indicates the presence of some bioactive compound like Silane, dimethoxymethyl-, Silanediol, dimethyl-, Cyclotrisiloxane, hexamethyl-,Cyclotetrasiloxane, octamethyl-,Cyclopentasiloxane, decamethyl-,Azulene, Cyclohexasiloxane, dodecamethyl-, 2,4-Di-tert-butylphenol, Cyclooctasiloxane, hexadecamethyl- etc. Thus, the present study confirmed that C. striata epidermal mucus is having potentials of antibacterial activity. However, the study offers the baseline information for the pharmaceutical industry's new medicine development.