Antimicrobial resistance (AMR) poses a significant threat to global health, with Enterococcus species emerging as prominent contributors due to their intrinsic resilience and adaptability. India, like many other countries, faces escalating rates of AMR, particularly concerning Enterococcal infections. However, limited regional data hinder targeted interventions. This study aimed to investigate the antimicrobial resistance profiles, species distribution, and virulence characteristics of Enterococcus species in central India. A cross-sectional study involving 64 clinical isolates was conducted over two years. Enterococcus faecalis predominated, with urine samples being the primary source. Antimicrobial susceptibility testing revealed high resistance to penicillin and ampicillin, while vancomycin remained effective. Virulence factors such as hemolysin, gelatinase, and biofilm production were prevalent. Comparative analysis with existing literature highlighted consistent resistance patterns and species distribution, emphasizing the need for tailored interventions. This study provides valuable insights into combating AMR and improving infection control strategies against Enterococcus infections in the Indian healthcare context